Genotyping and primer details


Genotyping and primer details for 722D05 - At4g02075
Line ID 722D05
Locus At4g02075 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:914127 of line 722D05 Chr4:914127
   
Insertion allele amplicon 8474 - 23vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23vd: 5'-CAATCTACCATTCAGTTGTGTCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:914622
   
wt amplicon (over InsPos) 50wd - 23vd
wt amplicon start Chr4:913540
wt amplicon end Chr4:914622
wt amplicon size 1083 bp
Locus specific primer 23vd: 5'-CAATCTACCATTCAGTTGTGTCGT-3'
2nd locus specific primer 50wd: 5'-CGTTAATTTGAAGGTTTAAGCCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11