Genotyping and primer details


Genotyping and primer details for 723F03 - At2g38070
Line ID 723F03
Locus At2g38070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15930187 of line 723F03 Chr2:15930187
   
Insertion allele amplicon 8474 - 24vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24vd: 5'-GATGACCCTCGTTACTCTTTTGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15929724
   
wt amplicon (over InsPos) 42vx - 24vd
wt amplicon start Chr2:15929724
wt amplicon end Chr2:15930793
wt amplicon size 1070 bp
Locus specific primer 24vd: 5'-GATGACCCTCGTTACTCTTTTGAG-3'
2nd locus specific primer 42vx: 5'-TACATTCATGACCAAAGAAGATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11