Genotyping and primer details


Genotyping and primer details for 723H09 - At1g44170
Line ID 723H09
Locus At1g44170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:16798687 of line 723H09 Chr1:16798687
   
Insertion allele amplicon 8474 - 47kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 47kh: 5'-ATGCCAATCTATCATTTGAAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16799114
   
wt amplicon (over InsPos) 16ky - 47kh
wt amplicon start Chr1:16798192
wt amplicon end Chr1:16799114
wt amplicon size 923 bp
Locus specific primer 47kh: 5'-ATGCCAATCTATCATTTGAAAACC-3'
2nd locus specific primer 16ky: 5'-CATACGAAAAGGATAGTTCCAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11