Genotyping and primer details


Genotyping and primer details for 725A05 - At4g24770
Line ID 725A05
Locus At4g24770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12767058 of line 725A05 Chr4:12767058
   
Insertion allele amplicon 8474 - AO52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AO52: 5'-CCAAAAACAATTCCACACATAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12766759
   
wt amplicon (over InsPos) 59rd - AO52
wt amplicon start Chr4:12766759
wt amplicon end Chr4:12767408
wt amplicon size 650 bp
Locus specific primer AO52: 5'-CCAAAAACAATTCCACACATAAAA-3'
2nd locus specific primer 59rd: 5'-ATTTAGCTGCTTTCTTCTTGTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11