Genotyping and primer details


Genotyping and primer details for 726E10 - At3g60210
Line ID 726E10
Locus At3g60210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22252615 of line 726E10 Chr3:22252615
   
Insertion allele amplicon 8474 - 57jd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 57jd: 5'-CATTGTTGGAAAAGAAAGCTAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22252191
   
wt amplicon (over InsPos) 03jz - 57jd
wt amplicon start Chr3:22252191
wt amplicon end Chr3:22253213
wt amplicon size 1023 bp
Locus specific primer 57jd: 5'-CATTGTTGGAAAAGAAAGCTAAGG-3'
2nd locus specific primer 03jz: 5'-AGAGATTGGACCAGATGGAAGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11