Genotyping and primer details


Genotyping and primer details for 730C07 - At1g69430
Line ID 730C07
Locus At1g69430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26098787 of line 730C07 Chr1:26098787
   
Insertion allele amplicon 8474 - 41cy
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 41cy: 5'-CAACTCTTAAAGCAGGGACAAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26099083
   
wt amplicon (over InsPos) 68dy - 41cy
wt amplicon start Chr1:26098220
wt amplicon end Chr1:26099083
wt amplicon size 864 bp
Locus specific primer 41cy: 5'-CAACTCTTAAAGCAGGGACAAATC-3'
2nd locus specific primer 68dy: 5'-CTTCTTTTGATCTGTCCTGTTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11