Genotyping and primer details


Genotyping and primer details for 730G10 - At5g27410
Line ID 730G10
Locus At5g27410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9682460 of line 730G10 Chr5:9682460
   
Insertion allele amplicon 8474 - 31fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31fr: 5'-AAAAGTCAAACAAGAGAGCCTGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9682843
   
wt amplicon (over InsPos) 11gl - 31fr
wt amplicon start Chr5:9682154
wt amplicon end Chr5:9682843
wt amplicon size 690 bp
Locus specific primer 31fr: 5'-AAAAGTCAAACAAGAGAGCCTGAC-3'
2nd locus specific primer 11gl: 5'-ATGGACTACAGGAACTATGGGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11