Genotyping and primer details


Genotyping and primer details for 731G08 - At2g45030
Line ID 731G08
Locus At2g45030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18575848 of line 731G08 Chr2:18575848
   
Insertion allele amplicon 8474 - 45uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 45uv: 5'-TCCTTAGCTGTTCAACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18575230
   
wt amplicon (over InsPos) 78vl - 45uv
wt amplicon start Chr2:18575230
wt amplicon end Chr2:18576329
wt amplicon size 1100 bp
Locus specific primer 45uv: 5'-TCCTTAGCTGTTCAACCA-3'
2nd locus specific primer 78vl: 5'-ACTTTCAACTCGACCAGCATAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11