Genotyping and primer details


Genotyping and primer details for 732E02 - At5g49950
Line ID 732E02
Locus At5g49950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20320416 of line 732E02 Chr5:20320416
   
Insertion allele amplicon 8474 - 18hg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18hg: 5'-TCGGTTCTCTTATGTTGTAGCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20321054
   
wt amplicon (over InsPos) 16ig - 18hg
wt amplicon start Chr5:20320008
wt amplicon end Chr5:20321054
wt amplicon size 1047 bp
Locus specific primer 18hg: 5'-TCGGTTCTCTTATGTTGTAGCCTT-3'
2nd locus specific primer 16ig: 5'-CTCTCTTCTTCAAAGGACCGAGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11