Genotyping and primer details


Genotyping and primer details for 733H02 - At4g03540
Line ID 733H02
Locus At4g03540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1570787 of line 733H02 Chr4:1570787
   
Insertion allele amplicon 8474 - 19aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19aq: 5'-AGAGAGCTTCCTTCTTAGCCATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1570139
   
wt amplicon (over InsPos) 19xd - 19aq
wt amplicon start Chr4:1570139
wt amplicon end Chr4:1571395
wt amplicon size 1257 bp
Locus specific primer 19aq: 5'-AGAGAGCTTCCTTCTTAGCCATCT-3'
2nd locus specific primer 19xd: 5'-GCAATTAAAGCTCCAGTGATCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11