Genotyping and primer details


Genotyping and primer details for 735D06 - At2g45850
Line ID 735D06
Locus At2g45850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18872005 of line 735D06 Chr2:18872005
   
Insertion allele amplicon 8474 - 14um
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14um: 5'-AATCAGAATTGCAAGCAAATTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18871627
   
wt amplicon (over InsPos) 25vl - 14um
wt amplicon start Chr2:18871627
wt amplicon end Chr2:18872231
wt amplicon size 605 bp
Locus specific primer 14um: 5'-AATCAGAATTGCAAGCAAATTACA-3'
2nd locus specific primer 25vl: 5'-CCTTTAATAGCTGCAAGTCCTGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11