Genotyping and primer details


Genotyping and primer details for 736A02 - At3g50910
Line ID 736A02
Locus At3g50910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18921522 of line 736A02 Chr3:18921522
   
Insertion allele amplicon 8474 - 57sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 57sm: 5'-CCTCTGGTATCAACAGAGTTGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18921847
   
wt amplicon (over InsPos) 28tc - 57sm
wt amplicon start Chr3:18920983
wt amplicon end Chr3:18921847
wt amplicon size 865 bp
Locus specific primer 57sm: 5'-CCTCTGGTATCAACAGAGTTGTGA-3'
2nd locus specific primer 28tc: 5'-AATCATTTCCTTATGAGATGGCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11