Genotyping and primer details


Genotyping and primer details for 737F10 - At2g24440
Line ID 737F10
Locus At2g24440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10392000 of line 737F10 Chr2:10392000
   
Insertion allele amplicon 8474 - NL56
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NL56: 5'-AAACGAATTGCTTCAAACTAGCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10392750
   
wt amplicon (over InsPos) 04bj - NL56
wt amplicon start Chr2:10391698
wt amplicon end Chr2:10392750
wt amplicon size 1053 bp
Locus specific primer NL56: 5'-AAACGAATTGCTTCAAACTAGCAT-3'
2nd locus specific primer 04bj: 5'-TTGTTTGTTGTTGTTTTCCTGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11