Genotyping and primer details


Genotyping and primer details for 739A08 - At5g67200
Line ID 739A08
Locus At5g67200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26815557 of line 739A08 Chr5:26815557
   
Insertion allele amplicon 8474 - EU52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU52: 5'-AACCCTTGAGATTGAAGAAGATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26815078
   
Insertion allele amplicon CR3S - TN20
T-DNA primer CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Locus specific primer TN20: 5'-CGTTACTCCGACTCTATCACGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26815935
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11