Genotyping and primer details


Genotyping and primer details for 740A10 - At4g26860
Line ID 740A10
Locus At4g26860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13504672 of line 740A10 Chr4:13504672
   
Insertion allele amplicon 8474 - CR70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CR70: 5'-TAATACCTTTTCTCCATCCACACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13504095
   
wt amplicon (over InsPos) 09nv - CR70
wt amplicon start Chr4:13504095
wt amplicon end Chr4:13505157
wt amplicon size 1063 bp
Locus specific primer CR70: 5'-TAATACCTTTTCTCCATCCACACC-3'
2nd locus specific primer 09nv: 5'-AGACTTTTACAAACCCACAAATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11