Genotyping and primer details


Genotyping and primer details for 740F11 - At2g42670
Line ID 740F11
Locus At2g42670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17772500 of line 740F11 Chr2:17772500
   
Insertion allele amplicon 8474 - 52fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 52fr: 5'-CTGATTGGTCTTGAATGCTGTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17773118
   
wt amplicon (over InsPos) 17go - 52fr
wt amplicon start Chr2:17772127
wt amplicon end Chr2:17773118
wt amplicon size 992 bp
Locus specific primer 52fr: 5'-CTGATTGGTCTTGAATGCTGTTTA-3'
2nd locus specific primer 17go: 5'-TCCAGTTCACAATCCAAATCAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11