Genotyping and primer details


Genotyping and primer details for 741B04 - At1g80400
Line ID 741B04
Locus At1g80400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30226098 of line 741B04 Chr1:30226098
   
Insertion allele amplicon 8474 - 31uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31uv: 5'-CGAGAGCTAGAGTTGAATTTGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30225774
   
wt amplicon (over InsPos) 77vl - 31uv
wt amplicon start Chr1:30225774
wt amplicon end Chr1:30226464
wt amplicon size 691 bp
Locus specific primer 31uv: 5'-CGAGAGCTAGAGTTGAATTTGCTT-3'
2nd locus specific primer 77vl: 5'-CAGTGCTATTTTCTTCATCTGCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11