Genotyping and primer details


Genotyping and primer details for 741D05 - At4g34290
Line ID 741D05
Locus At4g34290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16411484 of line 741D05 Chr4:16411484
   
Insertion allele amplicon 8474 - 07gj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07gj: 5'-TCGCAGAGTATATCCCTCTTGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16412037
   
wt amplicon (over InsPos) 07hv - 07gj
wt amplicon start Chr4:16410859
wt amplicon end Chr4:16412037
wt amplicon size 1179 bp
Locus specific primer 07gj: 5'-TCGCAGAGTATATCCCTCTTGTTT-3'
2nd locus specific primer 07hv: 5'-CCACTCTCATACACAGTCACAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11