Genotyping and primer details


Genotyping and primer details for 742F07 - At4g17210
Line ID 742F07
Locus At4g17210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9653929 of line 742F07 Chr4:9653929
   
Insertion allele amplicon 8474 - BH56
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BH56: 5'-AGTTCAGCTAGTCTCTCTTCCACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9653606
   
wt amplicon (over InsPos) 26sq - BH56
wt amplicon start Chr4:9653606
wt amplicon end Chr4:9654458
wt amplicon size 853 bp
Locus specific primer BH56: 5'-AGTTCAGCTAGTCTCTCTTCCACG-3'
2nd locus specific primer 26sq: 5'-CCCCAACAAAAGAAAACAAGATTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11