Genotyping and primer details


Genotyping and primer details for 743H12 - At5g17200
Line ID 743H12
Locus At5g17200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5654599 of line 743H12 Chr5:5654599
   
Insertion allele amplicon 8474 - CE87
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CE87: 5'-CAAGTCTTCGACCATCTCCTTATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5654966
   
Insertion allele amplicon 8474 - CE88
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CE88: 5'-CGAAATCTCCAGTTACTCCAAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5654113
   
wt amplicon (over InsPos) CE88 - CE87
wt amplicon start Chr5:5654113
wt amplicon end Chr5:5654966
wt amplicon size 854 bp
Locus specific primer CE87: 5'-CAAGTCTTCGACCATCTCCTTATC-3'
2nd locus specific primer CE88: 5'-CGAAATCTCCAGTTACTCCAAGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11