Genotyping and primer details


Genotyping and primer details for 744A09 - At1g65220
Line ID 744A09
Locus At1g65220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24228354 of line 744A09 Chr1:24228354
   
Insertion allele amplicon 8474 - 59lm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 59lm: 5'-CGCTTAATACTGATATGATTGCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24227867
   
wt amplicon (over InsPos) 09mj - 59lm
wt amplicon start Chr1:24227867
wt amplicon end Chr1:24228987
wt amplicon size 1121 bp
Locus specific primer 59lm: 5'-CGCTTAATACTGATATGATTGCCA-3'
2nd locus specific primer 09mj: 5'-ACCTCACAAAGAGGTTAACCAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11