Genotyping and primer details


Genotyping and primer details for 744H05 - At1g03740
Line ID 744H05
Locus At1g03740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:935825 of line 744H05 Chr1:935825
   
Insertion allele amplicon 8474 - 30ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30ve: 5'-GTTAGTTACCTCCACGGTCATTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:936156
   
wt amplicon (over InsPos) 40vx - 30ve
wt amplicon start Chr1:935180
wt amplicon end Chr1:936156
wt amplicon size 977 bp
Locus specific primer 30ve: 5'-GTTAGTTACCTCCACGGTCATTGT-3'
2nd locus specific primer 40vx: 5'-GACAACTTCTAAGTGGGCTTGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11