Genotyping and primer details


Genotyping and primer details for 745C07 - At5g47380
Line ID 745C07
Locus At5g47380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19223115 of line 745C07 Chr5:19223115
   
Insertion allele amplicon 8474 - 30au
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30au: 5'-ACTTATCACGGAGATTGAGTTGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19223547
   
Insertion allele amplicon 8474 - KA25
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KA25: 5'-TGAGCAGGTACACCATATGCTAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19222496
   
wt amplicon (over InsPos) 30au - KA25
wt amplicon start Chr5:19222496
wt amplicon end Chr5:19223547
wt amplicon size 1052 bp
Locus specific primer KA25: 5'-TGAGCAGGTACACCATATGCTAAG-3'
2nd locus specific primer 30au: 5'-ACTTATCACGGAGATTGAGTTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11