Genotyping and primer details


Genotyping and primer details for 747B10 - At3g10810
Line ID 747B10
Locus At3g10810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3383052 of line 747B10 Chr3:3383052
   
Insertion allele amplicon 8474 - 44sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 44sj: 5'-CTACTATCACTGCTGTTTGGCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3382486
   
wt amplicon (over InsPos) 29tf - 44sj
wt amplicon start Chr3:3382486
wt amplicon end Chr3:3383283
wt amplicon size 798 bp
Locus specific primer 44sj: 5'-CTACTATCACTGCTGTTTGGCAAG-3'
2nd locus specific primer 29tf: 5'-TTGTTTCTGATTCGTTTTGTCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11