Genotyping and primer details


Genotyping and primer details for 750A10 - At5g59550
Line ID 750A10
Locus At5g59550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23999110 of line 750A10 Chr5:23999110
   
Insertion allele amplicon 8474 - BG40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BG40: 5'-TAGAAAACGAAGCACCAATCTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23998331
   
Insertion allele amplicon 8474 - BG41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BG41: 5'-CACGTTTTCATTTTCTCATCTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23999751
   
wt amplicon (over InsPos) 09tf - BG40
wt amplicon start Chr5:23998331
wt amplicon end Chr5:23999424
wt amplicon size 1094 bp
Locus specific primer BG40: 5'-TAGAAAACGAAGCACCAATCTTTT-3'
2nd locus specific primer 09tf: 5'-GATCAATAACAACCGTAGATCCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11