Genotyping and primer details


Genotyping and primer details for 750G07 - At3g47060
Line ID 750G07
Locus At3g47060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17335421 of line 750G07 Chr3:17335421
   
Insertion allele amplicon 8474 - 25ey
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25ey: 5'-GCTTAATTTCTGACAACACCACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17335800
   
wt amplicon (over InsPos) 06ho - 25ey
wt amplicon start Chr3:17335078
wt amplicon end Chr3:17335800
wt amplicon size 723 bp
Locus specific primer 25ey: 5'-GCTTAATTTCTGACAACACCACAA-3'
2nd locus specific primer 06ho: 5'-GTTGTTACGGTTGGTAGAATGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11