Genotyping and primer details


Genotyping and primer details for 751A12 - At2g32670
Line ID 751A12
Locus At2g32670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13858094 of line 751A12 Chr2:13858094
   
Insertion allele amplicon 8474 - BE33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BE33: 5'-CCTTACCACCACCATATCTCTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13858633
   
Insertion allele amplicon 3144 - CB32
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CB32: 5'-TTCAAACTTTAAGCATCCACACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13857723
   
wt amplicon (over InsPos) CB32 - BE33
wt amplicon start Chr2:13857723
wt amplicon end Chr2:13858633
wt amplicon size 911 bp
Locus specific primer BE33: 5'-CCTTACCACCACCATATCTCTTGT-3'
2nd locus specific primer CB32: 5'-TTCAAACTTTAAGCATCCACACTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11