Genotyping and primer details


Genotyping and primer details for 751C01 - At4g26060
Line ID 751C01
Locus At4g26060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13216078 of line 751C01 Chr4:13216078
   
Insertion allele amplicon 8474 - 03rg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03rg: 5'-TGTTATCTCATCTCAAGACCATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13215738
   
Insertion allele amplicon 8474 - NL66
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NL66: 5'-AGAAAAGTCCATTCATTTTTCCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13216748
   
wt amplicon (over InsPos) 19gl - NL65
wt amplicon start Chr4:13215355
wt amplicon end Chr4:13216397
wt amplicon size 1043 bp
Locus specific primer NL65: 5'-AGAGGCTTGTCATACACTCCATTT-3'
2nd locus specific primer 19gl: 5'-TTCCAGATTTCTTGATCCGATATT-3'
   
wt amplicon (over InsPos) 03rg - NL66
wt amplicon start Chr4:13215738
wt amplicon end Chr4:13216748
wt amplicon size 1011 bp
Locus specific primer NL66: 5'-AGAAAAGTCCATTCATTTTTCCAA-3'
2nd locus specific primer 03rg: 5'-TGTTATCTCATCTCAAGACCATGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11