Genotyping and primer details


Genotyping and primer details for 751H10 - At5g20690
Line ID 751H10
Locus At5g20690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7003062 of line 751H10 Chr5:7003062
   
Insertion allele amplicon 8474 - 60wz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 60wz: 5'-CCTATTCGATTTGTCCGAATTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7002007
   
Insertion allele amplicon 8474 - EU55
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU55: 5'-CCTTTACCAATATTCGACACACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7003504
   
wt amplicon (over InsPos) EU55 - TN23
wt amplicon start Chr5:7002478
wt amplicon end Chr5:7003504
wt amplicon size 1027 bp
Locus specific primer TN23: 5'-GTTTCTTTCTCCTCATCCTCCTCT-3'
2nd locus specific primer EU55: 5'-CCTTTACCAATATTCGACACACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11