Genotyping and primer details


Genotyping and primer details for 752H04 - At3g15890
Line ID 752H04
Locus At3g15890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5374681 of line 752H04 Chr3:5374681
   
Insertion allele amplicon 8474 - 70ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 70ns: 5'-AACACTCAACAAATTCTTGTGACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5375250
   
wt amplicon (over InsPos) 31oj - 70ns
wt amplicon start Chr3:5374251
wt amplicon end Chr3:5375250
wt amplicon size 1000 bp
Locus specific primer 70ns: 5'-AACACTCAACAAATTCTTGTGACG-3'
2nd locus specific primer 31oj: 5'-TATCAATCTCACATCGAGACGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11