Genotyping and primer details


Genotyping and primer details for 754F06 - At1g61240
Line ID 754F06
Locus At1g61240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22584227 of line 754F06 Chr1:22584227
   
Insertion allele amplicon 8474 - 16dh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16dh: 5'-AACACACACATTACACTCCTGCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22584851
   
wt amplicon (over InsPos) 71dy - 16dh
wt amplicon start Chr1:22583625
wt amplicon end Chr1:22584851
wt amplicon size 1227 bp
Locus specific primer 16dh: 5'-AACACACACATTACACTCCTGCTC-3'
2nd locus specific primer 71dy: 5'-TGAAGATCTTGGAGTGGAGAATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11