Genotyping and primer details


Genotyping and primer details for 760B09 - At2g17800
Line ID 760B09
Locus At2g17800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7741707 of line 760B09 Chr2:7741707
   
Insertion allele amplicon 8474 - 34st
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 34st: 5'-AACTAGGCCTGGATTTTGGAGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7741152
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11