Genotyping and primer details


Genotyping and primer details for 760D08 - At4g12650
Line ID 760D08
Locus At4g12650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7470075 of line 760D08 Chr4:7470075
   
Insertion allele amplicon 8474 - 24lu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24lu: 5'-TTTTCTTGATTTTCGTTATTCGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7470652
   
wt amplicon (over InsPos) 09mo - 24lu
wt amplicon start Chr4:7469508
wt amplicon end Chr4:7470652
wt amplicon size 1145 bp
Locus specific primer 24lu: 5'-TTTTCTTGATTTTCGTTATTCGCT-3'
2nd locus specific primer 09mo: 5'-CATACTTAACACTGCAGGGAACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11