Genotyping and primer details


Genotyping and primer details for 760E02 - At1g63830
Line ID 760E02
Locus At1g63830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23687052 of line 760E02 Chr1:23687052
   
Insertion allele amplicon 8474 - 01di
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01di: 5'-CTCAGATACTCTCTTGCTGTGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23686654
   
wt amplicon (over InsPos) 19ep - 01di
wt amplicon start Chr1:23298843
wt amplicon end Chr1:23686654
wt amplicon size 1012 bp
Locus specific primer 01di: 5'-CTCAGATACTCTCTTGCTGTGCTG-3'
2nd locus specific primer 19ep: 5'-CTTGTAAATCCGGTTTGAGACTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11