Genotyping and primer details


Genotyping and primer details for 761F11 - At1g72110
Line ID 761F11
Locus At1g72110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27131461 of line 761F11 Chr1:27131461
   
Insertion allele amplicon 8474 - NL76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NL76: 5'-AGAGTTTTACAGAGCCCAATACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27131999
   
Insertion allele amplicon 8474 - NL77
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NL77: 5'-CTTACATCTGAATGAGAGGAACCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27130995
   
wt amplicon (over InsPos) 02jp - NL76
wt amplicon start Chr1:27130998
wt amplicon end Chr1:27131999
wt amplicon size 1002 bp
Locus specific primer NL76: 5'-AGAGTTTTACAGAGCCCAATACCA-3'
2nd locus specific primer 02jp: 5'-ACATCTGAATGAGAGGAACCCTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11