Genotyping and primer details


Genotyping and primer details for 762E02 - At4g04900
Line ID 762E02
Locus At4g04900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:2486895 of line 762E02 Chr4:2486895
   
Insertion allele amplicon 8474 - FQ63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FQ63: 5'-TGAAATAGAAATTGGGTTTCCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2487252
   
wt amplicon (over InsPos) 06uo - FQ63
wt amplicon start Chr4:2486323
wt amplicon end Chr4:2487252
wt amplicon size 930 bp
Locus specific primer FQ63: 5'-TGAAATAGAAATTGGGTTTCCAAC-3'
2nd locus specific primer 06uo: 5'-GCTTTTCTTCTTGTTATTTTTCGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11