Genotyping and primer details


Genotyping and primer details for 763B01 - At5g48770
Line ID 763B01
Locus At5g48770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19774710 of line 763B01 Chr5:19774710
   
Insertion allele amplicon 8474 - 17di
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17di: 5'-TGTATGATATTCAGTCGCTCTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19775080
   
wt amplicon (over InsPos) 72dy - 17di
wt amplicon start Chr5:19774141
wt amplicon end Chr5:19775080
wt amplicon size 940 bp
Locus specific primer 17di: 5'-TGTATGATATTCAGTCGCTCTTGG-3'
2nd locus specific primer 72dy: 5'-TCATTTCCGCTGAGGTCTAAGTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11