Genotyping and primer details


Genotyping and primer details for 767B03 - At1g63290
Line ID 767B03
Locus At1g63290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23473627 of line 767B03 Chr1:23473627
   
Insertion allele amplicon 8474 - 18di
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18di: 5'-GGGCTTTACTTGTACCCAAACATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23474072
   
wt amplicon (over InsPos) 74dy - 18di
wt amplicon start Chr1:23473272
wt amplicon end Chr1:23474072
wt amplicon size 801 bp
Locus specific primer 18di: 5'-GGGCTTTACTTGTACCCAAACATA-3'
2nd locus specific primer 74dy: 5'-TTTCACATTTGAATTCCACAACAT-3'
   
wt amplicon (over InsPos) 03wy - 18di
wt amplicon start Chr1:23473006
wt amplicon end Chr1:23474072
wt amplicon size 1067 bp
Locus specific primer 18di: 5'-GGGCTTTACTTGTACCCAAACATA-3'
2nd locus specific primer 03wy: 5'-CATTACTTTGTGTGCTTCCTCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11