Genotyping and primer details


Genotyping and primer details for 767B08 - At2g05310
Line ID 767B08
Locus At2g05310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:1933415 of line 767B08 Chr2:1933415
   
Insertion allele amplicon 8474 - 73ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 73ra: 5'-TCTGTTAATCCTTGGTTCTTGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:1933944
   
wt amplicon (over InsPos) 23rt - 73ra
wt amplicon start Chr2:1933065
wt amplicon end Chr2:1933944
wt amplicon size 880 bp
Locus specific primer 73ra: 5'-TCTGTTAATCCTTGGTTCTTGCTT-3'
2nd locus specific primer 23rt: 5'-TCTAAGCAATGTCAAGCTCTTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11