Genotyping and primer details


Genotyping and primer details for 769D04 - At1g53165
Line ID 769D04
Locus At1g53165 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19818782 of line 769D04 Chr1:19818782
   
Insertion allele amplicon 8474 - 24wm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24wm: 5'-TGGTTATTTCTGACATGAAAGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19818419
   
wt amplicon (over InsPos) 18xd - 24wm
wt amplicon start Chr1:19818419
wt amplicon end Chr1:19819819
wt amplicon size 1401 bp
Locus specific primer 24wm: 5'-TGGTTATTTCTGACATGAAAGCTG-3'
2nd locus specific primer 18xd: 5'-TGCTATTTCCAGAGTAACGATTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11