Genotyping and primer details


Genotyping and primer details for 772A03 - At5g24640
Line ID 772A03
Locus At5g24640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:8436757 of line 772A03 Chr5:8436757
   
Insertion allele amplicon 8474 - NL89
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NL89: 5'-ACATTAGCCCAGATATGCCAATAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:8436187
   
wt amplicon (over InsPos) 54rd - NL89
wt amplicon start Chr5:8436187
wt amplicon end Chr5:8437207
wt amplicon size 1021 bp
Locus specific primer NL89: 5'-ACATTAGCCCAGATATGCCAATAG-3'
2nd locus specific primer 54rd: 5'-GTTGAATTGGATTCTGTTGATCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11