Genotyping and primer details


Genotyping and primer details for 772C09 - At5g18130
Line ID 772C09
Locus At5g18130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5995521 of line 772C09 Chr5:5995521
   
Insertion allele amplicon 8474 - CS38
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CS38: 5'-TGAGTCAGAGTGTCTCTCATCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5996080
   
wt amplicon (over InsPos) 20ho - CS38
wt amplicon start Chr5:5995115
wt amplicon end Chr5:5996080
wt amplicon size 966 bp
Locus specific primer CS38: 5'-TGAGTCAGAGTGTCTCTCATCGTT-3'
2nd locus specific primer 20ho: 5'-TGTGGAAAATGAATTAGCGTGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11