Genotyping and primer details


Genotyping and primer details for 773B10 - At2g44350
Line ID 773B10
Locus At2g44350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18320085 of line 773B10 Chr2:18320085
   
Insertion allele amplicon 8474 - 60gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 60gp: 5'-GAGAATTTGCCTTGAAGCATCTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18319790
   
wt amplicon (over InsPos) 09hs - 60gp
wt amplicon start Chr2:18319790
wt amplicon end Chr2:18320601
wt amplicon size 812 bp
Locus specific primer 60gp: 5'-GAGAATTTGCCTTGAAGCATCTAC-3'
2nd locus specific primer 09hs: 5'-AAAAGCTATCCAGTGTGGAAGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11