Genotyping and primer details


Genotyping and primer details for 774D08 - At4g28770
Line ID 774D08
Locus At4g28770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14213476 of line 774D08 Chr4:14213476
   
Insertion allele amplicon 8474 - 23dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23dr: 5'-GGAAGTCTCTTCACAATGAGCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14213811
   
wt amplicon (over InsPos) 17ej - 23dr
wt amplicon start Chr4:14213099
wt amplicon end Chr4:14213811
wt amplicon size 713 bp
Locus specific primer 23dr: 5'-GGAAGTCTCTTCACAATGAGCTTT-3'
2nd locus specific primer 17ej: 5'-TAGCTTAACGCTTTGTCCTTTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11