Genotyping and primer details


Genotyping and primer details for 777A03 - At2g37900
Line ID 777A03
Locus At2g37900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15864476 of line 777A03 Chr2:15864476
   
Insertion allele amplicon 8474 - CS42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CS42: 5'-CGTTTTTCATCAAACAAGCAATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15865039
   
wt amplicon (over InsPos) 17ig - CS42
wt amplicon start Chr2:15864001
wt amplicon end Chr2:15865039
wt amplicon size 1039 bp
Locus specific primer CS42: 5'-CGTTTTTCATCAAACAAGCAATAA-3'
2nd locus specific primer 17ig: 5'-CTAAATCTTGCTTGGAGAAGGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11