Genotyping and primer details


Genotyping and primer details for 777C04 - At1g80090
Line ID 777C04
Locus At1g80090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30131852 of line 777C04 Chr1:30131852
   
Insertion allele amplicon 8474 - 35dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35dl: 5'-AACACTGTTTTAGCCCTCACTAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30131226
   
wt amplicon (over InsPos) 10ec - 35dl
wt amplicon start Chr1:30131226
wt amplicon end Chr1:30132255
wt amplicon size 1030 bp
Locus specific primer 35dl: 5'-AACACTGTTTTAGCCCTCACTAGC-3'
2nd locus specific primer 10ec: 5'-TCTCATTCGTATGTCATGCTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11