Genotyping and primer details


Genotyping and primer details for 777D11 - At5g10530
Line ID 777D11
Locus At5g10530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3326823 of line 777D11 Chr5:3326823
   
Insertion allele amplicon 8474 - BB07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BB07: 5'-AAAGTTTGACTCTGCTTGTTCACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3327302
   
Insertion allele amplicon 8474 - EU63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU63: 5'-AAACCCAATTGTAACTTCTGATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3326238
   
wt amplicon (over InsPos) BB07 - EU63
wt amplicon start Chr5:3326238
wt amplicon end Chr5:3327302
wt amplicon size 1065 bp
Locus specific primer EU63: 5'-AAACCCAATTGTAACTTCTGATGG-3'
2nd locus specific primer BB07: 5'-AAAGTTTGACTCTGCTTGTTCACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11