Genotyping and primer details


Genotyping and primer details for 779D05 - At5g55920
Line ID 779D05
Locus At5g55920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22645951 of line 779D05 Chr5:22645951
   
Insertion allele amplicon 8474 - 30ew
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30ew: 5'-CGTCGTGAATAAACAAAAACAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22645623
   
wt amplicon (over InsPos) 08fq - 30ew
wt amplicon start Chr5:22645623
wt amplicon end Chr5:22646300
wt amplicon size 678 bp
Locus specific primer 30ew: 5'-CGTCGTGAATAAACAAAAACAAGA-3'
2nd locus specific primer 08fq: 5'-CAACCATGCACTTGTCAATACTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11