Genotyping and primer details


Genotyping and primer details for 779E04 - At3g17940
Line ID 779E04
Locus At3g17940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6143749 of line 779E04 Chr3:6143749
   
Insertion allele amplicon 8474 - 62sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 62sm: 5'-TTAAGGGAGGACGAAGAGATTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6143309
   
wt amplicon (over InsPos) 26tc - 62sm
wt amplicon start Chr3:6143309
wt amplicon end Chr3:6144234
wt amplicon size 926 bp
Locus specific primer 62sm: 5'-TTAAGGGAGGACGAAGAGATTACA-3'
2nd locus specific primer 26tc: 5'-ACTCCCATCAACTTAGCTCAACAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11