Genotyping and primer details


Genotyping and primer details for 781D02 - At2g15000
Line ID 781D02
Locus At2g15000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:6482367 of line 781D02 Chr2:6482367
   
Insertion allele amplicon 8474 - NF25
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NF25: 5'-GTGCTCCTAGATTCCTGAAGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:6481976
   
wt amplicon (over InsPos) 75dy - NF25
wt amplicon start Chr2:6481976
wt amplicon end Chr2:6482753
wt amplicon size 778 bp
Locus specific primer NF25: 5'-GTGCTCCTAGATTCCTGAAGAAGA-3'
2nd locus specific primer 75dy: 5'-ATTACGGATTTACGTTTTTAGCGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11